One of these options, Capto adhere ImpRes, is a cost-effective and flexible chromatography medium (resin) designed for high-resolution polishing of MAbs.Ĭapto adhere ImpRes is a multimodal anion exchange medium with a ligand (Fig 1) that displays high selectivity compared with traditional ion exchange polishing media. After the initial protein A capture step, there is a wide range of options for intermediate and polishing purification steps. GE Healthcare Life Sciences’ MAb production toolbox employs protein A chromatography media such as MabSelect SuRe™ or MabSelect SuRe LX for capture of the target. A platform approach is desirable as it saves both time and money in process development. The relative homogeneity of MAbs makes them well-suited for platform processes, which are sets of unit operations, conditions, and methods applied to molecules of a given class. As a result, more cost-effective, efficient, and flexible process purification schemes are one of the highest priorities for MAb manufacturers. MAbs and MAb conjugates are today in great demand for use as biopharamaceuticals. The results showed high yields of monomeric MAb, as well as good clearance of aggregates, host cell proteins (HCP), and leached protein A. The effects of buffer, pH, conductivity, and sample load were investigated. The study presents results from optimization of the loading conditions using the Design of Experiments (DoE) approach. In this study, the binding capacity for MAbs and the efficiency in the clearance of impurities using Capto adhere ImpRes in bind/elute (B/E) mode was evaluated. Capto adhere ImpRes is a strong ion exchanger with multimodal functionality designed for polishing of monoclonal antibodies (MAbs).
0 kommentar(er)
